Fig. 2. Ir76b required for low salt preference.

(A) Genomic organization of Ir76b. Shown are a P-element (P) inserted in Ir76b, the deletions in Ir76b¹ and Ir76b², and the genomic region included in the Ir76b-Gal4. (B) Two-way choice tests to sucrose versus sucrose plus salt. The “rescue” were Ir76b¹ flies harboring the UAS-Ir76b and the Ir76b-Gal4 transgenes. n = 10. (C and D) 50 mM NaCl-induced action potentials in L4 and S6 sensilla. n = 15. (E and F) Action potential frequencies produced by L4 (n = 5) and S6 (n = 3) sensilla across different NaCl concentrations. ANOVA tests. (G) Cartoon depicting that loss of Ir76b selectively disrupted the attractive salt taste pathway. SEMs. *p < 0.01.