Fig. 2.

Elongation and association of Protofibrils (Ps). (A), Elongation of 2 μM Ps monitored over a period of two hours using ThT fluorescence in the presence of 25 μM Aβ42 monomers (circles). A similar reaction with 2 μM fibrils was used as negative control (triangles). (B), P association monitored over a period of 12 h using dynamic light scattering (DLS) in presence (light gray circles) and absence (gray circles) of 150 mM NaCl. The data is plotted against normalized count rates per second (Kcs^–1). (C and D), Size distribution of Ps after 2, 6, and 20 h of elongation with 25 μM Aβ42 monomer and after 2, 6, and 12 h of association with 150 mM NaCl respectively, monitored using DLS.