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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Mol Microbiol. 2010 Feb 4;75(5):1182–1198. doi: 10.1111/j.1365-2958.2009.07038.x

Fig. 2. VSP52 and Arp2/3 complex mutants do not form hyphae.

Fig. 2

A. Overnight cultures grown in YPD media were diluted 1:200 in YPD (yeast conditions) or YPD + 10% FBS (hyphae conditions) and placed for 3 h at either 30°C (yeast) or 37°C (hyphae). Both transposon and deletion mutants for VSP52 and ARP2 do not form hyphae under these conditions. Heterozgygous mutants and revertants behaved like WT. An arp2Δ/Δarp3Δ/Δ double knockout showed identical behaviour compared with the arp2Δ/Δ single knockout. Bar = 10 μm.

B. Quantifying polarized morphogenesis. Hyphal induction was done as described for (A). While > 96% of WT, heterozygous and revertant strains formed true hyphae, the majority of vps52Δ/Δ, arp2Δ/Δ and arp2Δ/Δarp3Δ/Δ mutants did not form hyphae and grew as pseudohyphae instead. n > 200 for each strain.