A. Cells in logarithmic phase were incubated for 5 min with 20 μM FM4-64, washed twice, chased for 45 min and visualized by epifluorescence microscopy. Under these conditions, the dye is endocytosed and reliably visualized by vacuolar membrane staining in WT (SN95) and mutant cells.
B. Logarithmically growing cells were incubated with 4 mg ml−1 Lucifer Yellow (LY), incubated for 90 min, washed twice and visualized. LY is endocytosed in both WT and Arp2/3 complex mutants. Bars = 10 μm.