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The nrg1Δ/Δarp2Δ/Δ mutant phenocopies arp2Δ/Δ cell morphologies. Hyphal formation was assayed as described in Fig. 2A. Deletion of one transcriptional repressor for hyphal-specific genes, NRG1, leads to constitutive filamentous growth even in the absence of any hyphal signals (yeast). Deleting NRG1 in the arp2Δ/Δ mutant (nrg1Δ/Δarp2Δ/Δ) did not restore filamentous growth even in the presence of hyphal signals (hyphae). Bar = 10 μm.
