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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Mol Microbiol. 2010 Feb 4;75(5):1182–1198. doi: 10.1111/j.1365-2958.2009.07038.x

Fig. 8. Arp2/3 complex mutants show typical actin patch-associated phenotypes.

Fig. 8

A. Plate spotting assays were done as described in Fig. 1 legend. Arp2/3 complex mutants show similar cell wall and cell membrane defects as well as salt sensitivity as previously described for myo5Δ/Δ mutants.

B. Arp2/3 complex mutants showed aberrant cell wall deposition, indicating defects in cell separation (arrows). See also movie 2 (Supporting information) with arp2Δ/Δ cells that show cell separation defects. Logarithmically growing cells were stained with CFW directly in YPD media for 5 min, washed and visualized. Bar = 10 μm.