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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Neuron. 2014 Jul 2;83(1):164–177. doi: 10.1016/j.neuron.2014.05.006

Figure 3. Activity in DSOG1 neurons controls consumption.

Figure 3

A. Controls and flies expressing Kir2.1 in DSOG1 before (pre-feeding) and after (post-feeding) water consumption. The genotype for DSOG1 inactivation was 98-Gal4, 276B-FLP, tub>Gal80>, tub-Gal80ts, UAS-Kir2.1. Flies were kept two days at 30°C for Kir2.1 induction or remained at 22°C for controls.

B. DSOG1 inactivation increased consumption in deprived (24h wet-starved for 1M sucrose and 1mM denatonium; 24h dry-starved for water) and non-deprived (fed) conditions. 100% ethanol (EtOH) and 6M sodium chloride (NaCl) were consumed by non-deprived animals. n=20; mean±SEM; one-way ANOVA, Tukey post-hoc, ***p<0.001. † flies died after consumption.

C. DSOG1 inactivation with shibirets (Shits) caused 1mM denatonium consumption in non-deprived animals. n=20; mean±SEM; one-way ANOVA, Tukey post-hoc, ***p<0.001.

D. dTRPA1 activation of DSOG1 (98-Gal4, 276B-FLP, tub>Gal80>, UAS-dTRPA1) reduced sucrose and water consumption in deprived flies. n=61-71; mean+/-s.e.m.; one-way ANOVA, square root transformation, Tukey post-hoc, ***p<0.001. See Figure S3 for additional feeding phenotypes.