Skip to main content
. 2014 Jul 10;9(7):e101575. doi: 10.1371/journal.pone.0101575

Figure 3. Kinetic characterization of STNhaA.

Figure 3

(a) Transient electrical currents generated by purified STNhaA reconstituted in proteoliposomes using SSM-based electrophysiology after 10 mM Na+ and Li+ concentration jumps at pH 8.5. The buffers contained 25 mM Tris/25 mM MOPS/25 mM HEPES pH 8.5 (Tris), 290 mM KCl and 0.1 mM DTT. In addition, the activating and the nonactivating solutions contained 10 mM NaCl and KCl, respectively. The dashed line shows inhibition of the NaCl concentration jump signal with 25 µM 2-aminoperimidine. (b) The pH dependence of STNhaA peak currents after a 100 mM Na+ concentration jump. The buffers contained 25 mM Tris/25 mM MOPS/25 mM HEPES at pH values indicated (HCl or Tris), 200 mM KCl and 0.1 mM DTT. In addition, the activating and the non-activating solutions contained 100 mM NaCl and KCl, respectively. The dashed line represents the corresponding pH dependence of EcNhaA [20], [21].