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. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743

Figure 4. Effects of iron limitation on levels of photosystem proteins and heme.

Figure 4

A, Immunoblot analysis of photosystem proteins. Thylakoid membrane proteins were separated by SDS-PAGE and blotted onto a nitrocellulose membrane. Proteins PsaC, PsaD, PsbA, PsbB, and IsiA were detected using specific antibodies. Each sample contained 20 µg of thylakoid membrane proteins. B, Heme staining of Cytochrome c550 using 3,3′,5,5′-tetramethylbenzidine (TMBZ). Each sample contained 20 µg of whole cell proteins. Densitometric analysis of immunoblot and heme staining results were shown on the left. Means±SD were calculated from three biological replicates. The density of bands was normalized to the values from the iron-replete wild-type cells, except for IsiA immunoblot. The density of bands in IsiA immunoblot was normalized to the values of the wild-type cells subjected to 24 hours of iron limitation.