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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Innate Immun. 2014 Jan 3;21(1):42–54. doi: 10.1177/1753425913516524

Figure 2. Optimal timepoints for neutrophil respiratory burst testing after Hem alone.

Figure 2

Blood was collected at three different timepoints (4, 12 and 24 hours) after Hem. Neutrophils were isolated and resuspended in 100 ul sterial HBSS, then added to 100 ul of 0.398% Cytochrome C and 20 nM of PMA (as activating agonists) for burst test. The blank control was set as no cells; the negative control was set as without 20 nM of PMA. Twelve hours after Hem is the optimum time to give TAT-SNAP-23. A typical result of three independent experiments is provided.