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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Innate Immun. 2014 Jan 3;21(1):42–54. doi: 10.1177/1753425913516524

Figure 3. TAT-SNAP-23 inhibits neutrophil respiratory burst in Hem model.

Figure 3

Hem mice were given 24 ug TAT-SNAP-23 in 200 ul HBSS/mouse, or vehicle (200 ul HBSS/mouse) by intravenous infusion during resuscitation period. Blood was collected 12 hours post surgery. Neutrophils were isolated and resuspended in 100 ul sterial HBSS, then added to 100 ul of 0.398% Cytochrome C and 20 nM of PMA (as activating agonists) for burst test. The blank control was set as no cells; the negative control was set as without 20 nM of PMA. 24 ug/mouse TAT-SNAP-23 significantly inhibits the neutrophil respiratory burst 12 hours after Hem comparing with vehicle-treated control group (n=6–8/group, *p<0.05 TAT-SNAP-23 vs. vehicle treatment group, determined by Unpaired student t test), All data are expressed as mean ± SEM.