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. 2014 Jul 10;9(7):e102067. doi: 10.1371/journal.pone.0102067

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© 2014 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Sequence alignment of SlSRN1 with NbNTP2 and AtNAC017. The numbers on the left indicate amino acid positions of the proteins used. Shared amino acid residues are shown in black background. Conserved NAC domain and putative transmembrane motif are indicated. B. Phylogenetic tree analysis of SlSRN1 with other plant NAC proteins. Phylogenetic tree was constructed by neighbour-joining method using MEGA program version 6.05. SlSRN1 in the tree is indicated by an arrow. Plant NAC proteins used and their GenBank accessions are as follows: Arabidopsis AtNAC017 (AAK32826), AtNAC016 (AAD39614) and AtNAC013 (AEE31534), Nicotiana benthamiana NbNTP2 (AGY49287), potato StNTP2 (AGY49285) a tomato predicted NAC (Solyc04g072220) and rice Os09g32040 (BAF25461).