Table 1. Oligonucleotide primer sequences used for qPCR assays.
Gene | Forward (F) or reverse (R) Primer | Sequence(5′-3′) | Amplicon size | Primer efficiency |
Pten | F | CACCTATTCCTCAGCCCTTAT | 273 bp | 0.99 |
R | AACCCTCATTCAGACCTTCAC | |||
Mapk | F | GTCGCCATCAAGAAAATCAGC | 309 bp | 1.05 |
R | GGAAGGTTTGAGGTCACGGT | |||
CyclinD1 | F | GGACCGCTTCCTGTCGCT | 204 bp | 0.89 |
R | GCCAGGTTCCACTTGAGTTTGT | |||
S6k1 | F | CTGGGTGAAGAATGGAAGGG | 101 bp | 0.99 |
R | CGAACTCTGCCATGGGTCA | |||
4Ebp1 | F | TTTGAGATGGACATTTAAAGGGC | 101 bp | 0.94 |
R | CTTGCATAAGGCCTGGCTG | |||
Elf5 | F | CACCTATTCCTCAGCCCTTAT | 273 bp | 0.92 |
R | AACCCTCATTCAGACCTTCAC | |||
Stat5 | F | GTCCCTTCCCGTGGTTGT | 614 bp | 1.00 |
R | CGGCCTTGAATTTCATGTTG | |||
Mtor | F | ATGCTGTCCCTGGTCCTTATG | 178 bp | 0.92 |
R | GGGTCAGAGAGTGGCCTTCAA | |||
Csn2 | F | CCATAACAGCCTCCCAC | 111 bp | 0.94 |
R | GCCATAGCCTCCTTCAC | |||
Srebp1 | F | CCAGCTGACAGCTCCATTGA | 67 bp | 0.97 |
R | TGCGCGCCACAAGGA | |||
Pparγ | F | TCAAAGTGGAGCCTGTATC | 138 bp | 0.95 |
R | CATAGTGGAACCCTGACG | |||
Glut1 | F | CTTCATCCCAGCCCTGTT | 193 bp | 1.00 |
R | GACCTTCTTCTCCCGCATC | |||
Akt | F | TAAAGAAGGAGGTCATCGTGG | 181 bp | 0.98 |
R | CGGGACAGGTGGAAGAAAA | |||
β-Actin | F | AAGGACCTCTACGCCAACACG | 249 bp | 0.94 |
R | TTTGCGGTGGACGATGGAG |