Figure 2. Increased effector/memory CD4⁺ T cells in T-TRAF3^−/− mice.

Splenocytes were stained for CD4, Foxp3, CD44 and CD62L. CD4⁺Foxp3⁻ T cells were gated for CD44 and CD62L analysis. (A) percentages, (B) numbers of naïve and (C) numbers of CD44^hiCD62L^l°^w effector/memory T cells are shown. Data represent 10 mice in each group. (D) and (E) Splenocytes were stimulated with PMA and ionomycin for 6 hr. Intracellular staining for cytokine production in CD4⁺ T cell subsets was performed. (E) Summarized data from three individual experiments. *p<0.01.