Figure 5. Defective homeostasis of CD8⁺ Tcm cells in the absence of TRAF3.

(A) and (B) Sorted CD8⁺ T cells were labeled with CFSE and stimulated with 60 ng/ml IL-15 for 72 hr. Cells were stained for CD8, CD44 and CD62L. The dilution of CFSE in different populations is shown in (A), and data from three experiments are summarized in (B). (C) and (D) Freshly isolated splenocytes were stained for CD8, CD44, CD62L and Annexin V. Different CD8⁺ T cell subsets were gated for Annexin V analysis (C and D). Data represent 6 mice per group. (E) and (F) Splenocytes cultured for 24 hr without stimulation and stained for CD8, CD44, CD62L and Annexin V. Annexin V positive cells in different CD8⁺ T cell subsets are shown (E and F). Data represent one of three individual experiments. *p<0.01.