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. 2014 Jul 10;9(7):e102025. doi: 10.1371/journal.pone.0102025

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Lymphocytes were gated according to forward and side scatter (R1), (B) CD4⁺CD25⁺ lymphocytes (R2), CD4⁺CD25⁻ lymphocytes (R3). From the gated lymphocyte population, the total Foxp3 expression and isotype control were analyzed (C) (R2) and (D) (R3).