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. 2014 Jul 10;10(7):e1004253. doi: 10.1371/journal.ppat.1004253

Figure 3. LANA up-regulated Id1 transcription in a BMP-Smad1 dependent manner.

Figure 3

(A) Noggin inhibited Id1 up-regulation in LANA transfected 293T cells. LANA or vector transfected cells (2 µg each) were treated with noggin for 6 hours before harvest. Data were shown as mean ± s.e.m., n = 3. (B) BMP2 further enhanced LANA induction of Id1 expression. LANA or vector transfected cells (2 µg each) were treated with BMP2 for indicated times before harvest. Data were shown as mean ± s.e.m., n = 3. (C) LANA up-regulated activity of Id1-985 but not Id1-956. A Smad1 binding site was contained in Id1-985 promoter region, but not in Id1-956. Data were shown as mean ± s.e.m., n = 3. (D) LANA did not up-regulate Id1-985 activity in 293T-shSmad1 cells. Data were shown as mean ± s.e.m., n = 3. (E) LANA was recruited to Id1 promoter. SF-LANA and HA-Smad1 were co-transfected into 293T cells (12 µg each). Cells were treated with BMP2 for 2 hours before ChIP assay using indicating antibodies. (F) LANA significantly enhanced the enrichment of Smad1 at Id1 promoter. HA-Smad1 was co-transfected with SF-LANA or vector (12 µg each). Cells were treated with BMP2 for 2 hours before ChIP assay using HA antibody.