(A) FACS analysis of IL-17A and IFN-γ expression in CD4+CD44+ cells from Jarid2fl/fl; CD4-cre mice and littermate controls cultured under Th17 polarizing conditions without IL-1β and (B) the corresponding percentages of CD4+CD44+IL-17A+ T cells. (C) Relative expression of Il17a, Il17f, Atf3, Rorc, Irf4, Batf mRNA by RT-qPCR from WT and Jarid2-deficient Th17 cultures. (D) Relative expression of Il22, Il9 and Il10 mRNA in Jarid2-deficient and WT Th17 cultures was determined by RT-qPCR. (E) Cytokine concentrations in supernatant of Th17 cultures were determined by ELISA. (F) H3K27me3 at promoters of Il22, Il10, Il9 and Hprt determined by ChIP-qPCR. (G) RT-qPCR of Il22 intron 4 and Il9 intron 3 of Jarid2-deficient and WT Th17 cultures ± reverse transcriptase (RT).