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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Inhal Toxicol. 2012 Dec;24(14):995–1008. doi: 10.3109/08958378.2012.745633

Figure 8.

Figure 8

Photomicrographs of alveolar macrophages (AM) stained with acridine orange (lysosomal marker) and exposed to low or high nickel containing multi-walled carbon nanotubes (MWCNT). Freshly isolated AM from C57BL/6 mice were incubated in 96-well glass-bottom plates at 100μl/well of 106 cells/ml with 50 μg/ml of either low nickel (FA04) or high nickel (FA21) MWCNT for 2 h. The cells were co-stained with acridine orange (1:1000) to visualize phagolysosomes and photomicrographs were taken as described in Methods section. (A) Organized lysosomal formation visible in FA04- (low nickel MWCNT) exposed cells. (B) FA21- (high nickel MWCNT) exposed cells show very few phagolysosomes indicated by lack of green stain. All images at 600× using oil immersion objectives.