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. 2014 Jun 30;15(1):544. doi: 10.1186/1471-2164-15-544

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© Possidonio et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Methyl-β-cyclodextrin enhances muscle differentiation. Chick myogenic cells were grown for 24 hours, treated with 2 mM of methyl-β-cyclodextrin (MbCD) for 30 minutes and grown for the next 24 hours. Untreated (A) and MbCD-treated cells (B) were fixed and double-stained with an anti-sarcomeric α-actinin antibody (green) and the nuclear dye DAPI (blue). Merged images are shown in A and B. Note the α-actinin distribution in Z-lines along sarcomeres in both untreated and MbCD-treated muscle cells (arrows in A and B). Scale bar in B represents 10 μm.