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. 2014 Jun 30;15(1):544. doi: 10.1186/1471-2164-15-544

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© Possidonio et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Lim domain only protein 7 (LMO7) localizes at the nuclei region of chick myotubes. Chick myogenic cells were grown for 48 hours and double-stained with an anti-LMO7 antibody (green, A, B, D) and the nuclear dye DAPI (blue, C, D). B is an inset of a region of image A. A merged image is shown in D. Note the strong nuclear envelope staining of the anti-LMO7 antibody in multinucleated myotubes (arrows in A), plus a diffuse cytoplasmic fibrillar staining in both mononucleated and multinucleated cells. Scale bar in C represents 20 μm.