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. 2014 Jun 24;15:517. doi: 10.1186/1471-2164-15-517

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© Zhang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Verification of 10 detected CNVRs by qPCR. CNVR1-10 represents 10 CNVRs selected for qPCR validation. X-axis shows the individuals used in the validation experiment. Y-axis shows the relative quantification (RQ) values obtained by qPCR. Different shapes denote samples with different RQ values. Asterisk: samples with RQ values about 1 denote normal individuals (two copies); Box: samples with RQ values below 0.59 (ln^1.5) denote copy number loss individuals; Triangle: samples with RQ values about 1.59 (ln³) or more denote copy number gain individuals (>three copies) [61].