Table 1.
Forward primers | Reverse primers | Taqman probes | |
---|---|---|---|
IL-6 | 5′GCTTAATTACACATGTTCTCTGGGAAA3′ | 5′CAAGTGCATCATCGTTGTTCATAC3′ | 5′ATCAGAATTGCCATTGCACAACTCTTTTCTCAT′3 |
PDK4 | 5′GGAAGTATCGACCCAAACTGTGA′3 | 5′GGTCGCAGAGCATCTTTGC3′ | 5′CACTCAAAGGCATCTTGGACTACTGCTACCA3′ |
PDP1 | 5′CGGGCACTGCTACCTATCCTT′3 | 5′ACAATTTGGACGCCTCCTTACT3′ | 5′AGTGGCACAAGCACCCCAATGATTACTTC3′ |
Sequences of forward and reverse primers as well as Taqman probes used for real-time PCR
IL-6 interleukin 6, PDK4 pyruvate dehydrogenase kinase 4, PDP1 pyruvate dehydrogenase phosphatase 1