Figure 2. Response of ABA related factors to BRC1 induction. mRNA levels of ABF3 and ABI5 were measured by qPCR as described in González-Grandío et al. (2013). Estradiol-inducible BRC1 plants and estradiol-inducible GUS plants (control) were treated with 20 µM estradiol for 8 h. Error bars are SEM of 3 biological replicates of 8 plants each. Asterisks are significant differences (Student t­-test, P < 0.05). Primers used were CCAAAGAGCG CCCTGGAT and TTTTCTCACT CGCCCAAACA T for ABF3, and CACCAGTTCA GGCAGGTGTT T and TGTCCACCCG CTCCAAA for ABI5. SAND was used as a reference gene.¹⁶