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. 2014 Jul 11;4:5637. doi: 10.1038/srep05637

Figure 3. Apoptosis and necroptosis pathways could be converted to each other when one was inhibited.

Figure 3

(a) Cellular proteins were extracted after hypoxia for 24 hours in cultured H9c2 cells. Biotin switch method followed by Western blotting showed that Necrostatin-1 (nec-1, 100 μM), a specific blocker of necroptosis increased the level of cleaved caspase3. PEDF and 44mer could decrease the level of cleaved caspase3 in nec-1 group (n = 4; *P < 0.05 compare to corresponding hypoxia group and nec-1 group). (b) Z-VAD-FMK (20 μM), a cell permeable pan caspase inhibitor, could increase the RIP3 expression after 24 h hypoxia. PEDF and 44mer could also decrease the level of RIP3 in Z-VAD-FMK group. Blots were cropped and full length blots appear in the supplementary information. (n = 4; *P < 0.05 compare to corresponding hypoxia group and Z-VAD-FMK group). Data are represented as means ± S.E.M.