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Flowchart indicating typical reconstruction steps with either method. BH reconstructions take longer due to histology and require multiple setups for recording and reconstruction with Neurolucida. As FI can be monitored online during 2PLSM image acquisition, there is in effect a 100% yield of complete reconstructions, whereas with BH, histological processing occasionally fails or is incomplete, in our hands giving a yield of around 50–80% (see main text).
