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. 2014 Apr 29;3(2):363–385. doi: 10.3390/cells3020363

Figure 5.

Figure 5

Chloroquine causes TfR, but not VEGFR2, accumulation in endosomes. A direct recycling assay was performed using the goat anti-VEGFR2 extracellular domain or mouse anti-TfR (red). Samples were fixed, permeabilised and labelled with either rabbit anti-EEA1 (green) or rabbit anti-cathepsin D (green). Primary antibodies were visualised using either FITC anti-rabbit IgG or AlexaFluor-488-conjugated anti-rabbit IgG, and the nucleus was seen with DAPI (blue). The images are 2D projections of a stacked series of 15–30 μm optical sections taken using a wide field deconvolution microscopy system. Insets show a two-fold magnification of the indicated region. Bar: 10 μm.