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. 2014 May 1;124(6):2396–2409. doi: 10.1172/JCI69073

Figure 4. Inactivation of endothelial HIF promotes IRI-associated tubulointerstitial fibrosis.

Figure 4

(A) Representative images of Sirius red–stained kidneys from EC-specific Hif1aHif2a–/– and Cre– control mice at day 9 after IRI. Right panel shows quantification of Sirius red–positive area in cortex and medulla (n = 4). (B) RT-PCR analysis of Col18a1, Loxl-2, Tgfb1, and Col1a1 mRNA levels in mutant and control kidneys at day 9 following IRI (n = 4). (C and D) Representative images of CD31- and F4/80-stained kidneys analyzed on day 9 after renal clamping. Right panels: quantification of CD31-positive area and F4/80-positive cell number/hpf (n = 4–5). Graph bars represent mean values ± SEM; *P < 0.05; **P < 0.01. Scale bars: 100 μm.