Table 1.

Selected candidate reference genes used in the qRT-PCR assay indicating name, GenBank accession number, annealing temperature (T_a), PCR efficiency, slope, regression coefficient and primers used for the expression study

Gene	Full gene name	GenBank accession number	Ta (°C)	PCR efficiency (%)	Slope	Regression coefficient (r2)	Forward primer (5′-3′)	Reverse primer (5′-3′)
ACTB	Beta-actin	DQ845171	62	99.66	−3.33	0.998	TGCCCTGAGGCTCTCTTCCA	TGCGGATGTCGACGTCACA
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	AF017079	62	96.84	−3.40	0.998	GCAAGTTCCACGGCACAGTC	CCCACTTGATGTTGGCAGGA
TBP	TATA box-binding protein	DQ845178	62	94.92	−3.45	0.993	AAAGACCATTGCACTTCGTGC	ATATCAGTGCGGTGGTGCG
HPRT1	Hypoxanthine phosphoribosyltransferase 1	DQ845175	60	102.21	−3.27	0.998	ATTATGGACAGGACCGAACGG	CCAACAGGTCGGCAAAGAACT
YWHAZ	Tyrosine 3-monooxygenase/tryptophan 5-Monooxygenase activation protein zeta polypeptide	DQ845179	60	98.84	−3.35	0.999	CCAACGCTTCACAAGCAGAGA	TGCTTGCTGTGACTGGTCCA
HMBS	Hydroxymethylbilane synthase mRNA	DQ845174	60	97.63	−3.39	0.998	AGCATGCCTTGGAGAGGAATG	CGCTTGCAGACAGCTCCAAT
18S	18 S ribosomal RNA	DQ222453.1	60	95.68	−3.43	0.993	CGGCTACCACATCCAAGGAA	GCTGGAATTACCGCGGCT
EEF1A2	Eukaryotic translation elongation factor 1 alpha 2	BC108110.1	60	101.78	−3.29	0.997	CGACTGGCCACCTCATCTACA	CCAGGCATACTTGAAGGAGCC