Table 5.
Effect of protein level and mangosteen peel pellets (Mago-pel) in concentrate diet on rumen total bacterial methanogen and predominant cellulolytic bacteria using real-time PCR technique
| Items | 16% CP
|
19% CP
|
SEM | Contrasts1
|
||||
|---|---|---|---|---|---|---|---|---|
| −MSP | +MSP | −MSP | +MSP | Pro | MSP | Pro×MSP | ||
| ------------------- copies/ml of rumen fluid -------------------- | ||||||||
| Total bacteria (×109) | 2.4 | 1.8 | 2.1 | 3.1 | 0.42 | NS | NS | NS |
| Methanogens (×106) | 1.1 | 1.1 | 1.2 | 1.3 | 0.13 | NS | NS | NS |
| F. succinogenes (×107) | 11.6 | 7.6 | 10.2 | 13.0 | 0.84 | NS | NS | NS |
| R. flavefaciens (×106) | 1.6 | 1.6 | 1.8 | 1.8 | 0.07 | * | NS | NS |
| R. albus (×106) | 2.6 | 2.5 | 2.6 | 2.6 | 0.08 | NS | NS | NS |
SEM = Standard error of the means, NS = Not significant,
*
p<0.05.
1
Pro = Protein, MSP = Mago-pel, Pro×MSP = Protein×Mago-pel, SEM = Standard error of the means, CFU = Colony forming unit, NS = No significant, *p<0.05.