Table 5.
Functional annotation clusters of up-regulated genes in uterine endometrium on D12 of pregnancy compared with those on D12 of the estrous cycle
| Annotation clustera | Enrichment scoreb | Biological termsc |
|---|---|---|
| 1 | 2.56 | Membrane fraction (12), cell fraction (14), insoluble fraction (12) |
| 2 | 1.04 | Endoplasmic reticulum part (6), endoplasmic reticulum membrane (5), endoplasmic reticulum (10), nuclear envelope-endoplasmic reticulum network (5), microsome (4), vesicular fraction (4), organelle membrane (9), endomembrane system (6) |
| 3 | 0.88 | Blood vessel morphogenesis (4), blood vessel development (4), vasculature development (4) |
| 4 | 0.86 | Cellular macromolecule catabolic process (9), proteolysis (11), macromolecule catabolic process (9), proteolysis involved in cellular protein catabolic process (7), cellular protein catabolic process (7), protein catabolic process (7), ubiquitin- protein ligase activity (3), small conjugating protein ligase activity (3), ligase activity, forming carbon-nitrogen bonds (3), ubiquitin-dependent protein catabolic process (3), modification-dependent macromolecule catabolic process (6), acid- amino acid ligase activity (3), modification-dependent protein catabolic process (6) |
| 5 | 0.80 | Intracellular organelle lumen (14), organelle lumen (14), nuclear lumen (10), membrane-enclosed lumen (14), nucleoplasm (8) |
| 6 | 0.72 | Proteolysis (11), metalloendopeptidase activity (3), endopeptidase activity (5), negative regulation of apoptosis (5), negative regulation of programmed cell death(5), negative regulation of cell death (5), metallopeptidase activity (3), peptidase activity, acting on L-amino acid peptides (5), peptidase activity (5), regulation of apoptosis (6), regulation of programmed cell death (6), regulation of cell death (6) |
| 7 | 0.72 | Phosphatase activity (5), phosphoprotein phosphatase activity (4), phosphate metabolic process (9), dephosphorylation (4), phosphorus metabolic process (9), protein amino acid dephosphorylation (3), protein serine/threonine kinase activity(3), phosphorylation (5), protein amino acid phosphorylation (5), protein kinase activity (3) |
| 8 | 0.71 | Positive regulation of multicellular organismal process (4), positive regulation of cell proliferation (5), regulation of growth (4) |
| 9 | 0.65 | Cell cycle (8), mitotic cell cycle (5), cell cycle process (5), cell cycle phase (4) |
| 10 | 0.65 | Glycosaminoglycan binding (3), polysaccharide binding (3), pattern binding (3), carbohydrate binding (3) |
Top ten of annotation clusters identified by official gene symbols of up-regulated gene list on D12 of pregnancy compared with those on D12 of the estrous cycle through DAVID.
Enrichment score represents significance of each cluster annotation and the relatedness of the terms and the genes associated with terms. Enrichment score is calculated by overall EASE scores (the modified Fisher extract p value) of each term member.
The gene ontology (GO) terms are group of terms having similar biological meaning with respect to biological process, cellular component, and molecular function. GO functional annotation term (FAT) was used for the analysis because GO term cannot universally define the specificity of a given term. The number in parenthesis indicate the number of differentially expressed genes contribute to the clustered term.