. 2012 Oct;25(10):1364–1373. doi: 10.5713/ajas.2012.12051

Table 5.

Effect of cassava hay and rice bran oil on rumen microorganism population in lactating dairy cows

Items	Control¹	CH	RBO	CHRBO	SEM	Contrast
Items	Control¹	CH	RBO	CHRBO	SEM	RBO	CH	Int.
Total viable count(CFU/ml)
Total bacteria (×10⁷)	2.59^a	3.13^a	1.71^b	2.71^a	2.52	^*	^*	NS
Cellulolytic (×10⁷)	1.36	2.60	1.26	2.13	5.00	NS	0.08	NS
Proteolytic (×10⁶)	4.40	6.32	4.22	5.52	0.96	NS	NS	NS
Amylolytic (×10⁶)	7.17	8.25	7.02	8.45	0.72	NS	NS	NS
Real-time PCR technique, copies/ml of rumen content
Total bacteria (×10¹⁰)	5.51^a	7.41^a	2.27^b	5.09^ab	0.85	^*	^*	NS
Anaerobic fungi (×10⁷)	0.93	2.57	1.46	1.93	0.70	NS	NS	NS
Methanogenes (×10⁷)	1.32	1.45	1.03	0.98	4.95	NS	NS	NS
F. succinogens (× 10⁹)	2.15^ab	5.29^a	0.97^b	3.06^ab	0.95	NS	^*	NS
R flavefaciens (×10⁷)	0.50^b	2.63^a	0.31^b	0.59^b	0.34	^*	^*	^*
R. albus (×10⁶)	3.01	2.56	3.16	4.48	1.35	NS	NS	NS
B. fibrisolvens (×10⁹)	1.34^a	1.80^ab	4.62^a	2.63^ab	1.00	^*	NS	NS
M elsdenii (×10⁸)	1.78^b	2.47^ab	5.08^a	3.60^ab	0.80	^*	NS	NS

Control = Non-supplementation with CH and RBO; CH = Concentrate with CH; RBO = Concentrate with 4% RBO; CHRBO = Concentrate with CH plus 4% RBO; Int. = Interaction. NS = non-significant difference (p>0.05).

p<0.05.

^a,b

Values on the same row with different superscripts differ (p<0.05). SEM = Standard error of the mean.