Table 1.
Ingredients and nutrient content of the experiment diets
| Diets | ||||||||
|---|---|---|---|---|---|---|---|---|
|
|
||||||||
| SLO | LO | SO | MIX | LL-HP | HL-SLO | HL-VO | LL-HC | |
| Ingredients (%) | ||||||||
| Fish meal1 | 50.0 | 50.0 | 50.0 | 50.0 | 63.0 | 50.0 | 50.0 | 52.0 |
| Wheat flour | 27.71 | 27.71 | 27.71 | 27.71 | 19.71 | 22.71 | 22.71 | 18.71 |
| Dehulled soybean meal | 7.0 | 7.0 | 7.0 | 7.0 | 7.0 | 7.0 | 7.0 | 7.0 |
| Alpha-starch | 11.0 | |||||||
| Wheat gluten | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| Beer yeast | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Squid liver oil | 5.0 | 1.0 | 10.0 | 1.0 | 1.0 | |||
| Linseed oil | 5.0 | 2.0 | 4.5 | |||||
| Soybean oil | 5.0 | 2.0 | 4.5 | |||||
| Vitamin premix2 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Mineral premix3 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Choline chloride | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| DL-α-tocopheryl acetate | 0.04 | 0.04 | 0.04 | 0.04 | 0.04 | 0.04 | 0.04 | 0.04 |
| Vitamin C4 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 |
| Nutrient contents (dry matter basis) | ||||||||
| Dry matter (%) | 74.9 | 77.5 | 75.9 | 77.3 | 72.8 | 79.6 | 77.7 | 73.2 |
| Crude protein (%) | 50.4 | 48.4 | 47.9 | 48.4 | 60.6 | 46.3 | 47.8 | 49.3 |
| Crude lipid (%) | 10.6 | 10.6 | 10.6 | 9.8 | 5.6 | 14.1 | 15.5 | 5.0 |
| Ash (%) | 9.6 | 9.7 | 9.7 | 9.6 | 11.9 | 9.7 | 9.5 | 9.8 |
| Energy (kcal/g) | 5.1 | 5.0 | 5.2 | 4.9 | 4.9 | 5.3 | 5.3 | 4.7 |
| n-3 HUFA5 | 3.4 | 2.5 | 2.6 | 2.4 | 1.8 | 4.6 | 3.9 | 1.6 |
Provided by Fisheries Co-op Feeds Co., Ltd. Gyeongsannam province, Korea.
Vitamin premix contained the following amount which was diluted in cellulose (g/kg premix): L-ascorbic acid, 121.2; DL-α-tocopheryl acetate, 18.8; thiamin hydrochloride, 2.7; riboflavin, 9.1; pyridoxine hydrochloride, 1.8; niacin, 36.4; Ca-D-pantothenate, 12.7; myo-inositol, 181.8; D-biotin, 0.27; folic acid, 0.68; p-aminobenzoic acid, 18.2; menadione, 1.8; retinyl acetate, 0.73; cholecalciferol, 0.003; cyanocobalamin, 0.003.
Mineral premix contained the following ingredients (g/kg premix): MgSO4·7H2O, 80.0; NaH2PO4·2H2O, 370.0; KCl, 130.0; Ferric citrate, 40.0; ZnSO4·7H2O, 20.0; Ca-lactate, 356.5; CuCl, 0.2; AlCl3·6H2O, 0.15; KI, 0.15; Na2Se2O3, 0.01; MnSO4·H2O, 2.0; CoCl2·6H2O, 1.0.
ROVIMIX® STAY-C® 35. DSM Nutrition Ltd. Seocho-ku, Korea.
Highly unsaturated fatty acids (C≥20), calculated (dietary total lipid %×area %×0.892). (Yoshimatsu et al., 1997).