. 2014 Jun;27(6):797–805. doi: 10.5713/ajas.2013.13560

Table 3.

pH, NH₃-N concentration (mg/100 mL) and microbial CP (mg/100 mL) at 4, 24, and 48 h after incubation

Feeds	pH			NH₃-N			Microbial CP

	4 h	24 h	48 h	4 h	24 h	48 h	4 h	24 h	48 h
CP levels in concentrate (%)
10	6.61	6.25	6.06	6.94^a	11.9^a	13.0^a	4.67^a	11.2^a	12.1^a
13	6.67	6.27	6.09	7.15^a	13.2^b	15.5^b	5.19^a	11.3^b	12.4^a
16	6.60	6.20	6.06	8.11^b	15.7^c	20.3^c	5.81^b	12.2^b	13.6^b
19	6.65	6.20	6.06	8.50^b	15.8^c	20.9^c	5.97^b	12.2^b	13.7^b
SEM	0.02	0.02	0.01	0.18	0.17	0.58	0.17	0.17	0.12
Contrast^d
L	ns	ns	ns	^***	^***	^***	^***	^**	^***
Q	ns	ns	ns	ns	^**	ns	ns	ns	ns
Roughage to concentrate ratios
80:20	6.77^a	6.30^a	6.16^a	5.26^a	11.2^a	13.4^a	4.58^a	8.07^a	10.9^a
60:40	6.75^a	6.28^ab	6.14^a	6.29^b	12.3^ab	15.1^ab	5.38^b	8.72^a	11.0^a
40:60	6.73^b	6.21^bc	6.11^b	6.51^b	13.3^b	16.7^b	5.51^b	9.26^a	11.9^b
20:80	6.66^c	6.19^c	6.08^c	7.20^c	15.8^c	20.1^c	5.77^c	11.4^b	13.0^c
SEM	0.01	0.03	0.01	0.14	0.56	0.56	0.10	0.48	0.14
Contrast
L	^***	^**	^***	^***	^***	^***	^***	^***	^***
Q	^***	ns	ns	ns	ns	ns	ns	ns	^**

^a,b,c

Values within a column with different superscript letters are significantly different (p<0.05).

L, linear; Q, quadratic; ns, non-significant.

^**

p<0.01,

^***

p<0.001.