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. 2014 May;27(5):635–647. doi: 10.5713/ajas.2013.13816

Figure 2.

Figure 2

Effect of rapamycin (R) treatment on the nuclear maturation of aged porcine oocytes. (A) Spindle position in control IVM (44 h; a), untreated 24 h-aged IVM (68 h; b–c), and 10 μM rapamycin-treated 24 h-aged IVM (44 h+10R/24 h; d) oocytes. (B) Percentage of aligned chromosomes and normalspindles in control IVM, untreated 24 h-aged IVM, and 10 μM rapamycin-treated 24 h-aged IVM oocytes. Significant differences from control oocytes are indicated (a–b p<0.05). Values are presented as means±SEM of independent experiments. α-Tubulin, green; chromatin, red. Bar = 40 μm. IVM, in vitro maturation; SEM, standard error of the mean.