Table 1.
Primers used for real-time PCR
| Gene | GenBank accession no. | Primer sequence | Annealing temp. (EC) | Product size (bp) |
|---|---|---|---|---|
| GAPDH | AF017079 | F:GGGCATGAACCATGAGAAGT R:AAGCAGGGATGATGTTCTGG |
60 | 230 |
| MOS | NM_001113219 | F:TGGGAAGAAACTGGAGGACA R:TTCGGGTCAGCCCAGGTTCA |
60 | 121 |
| BMP15 | NM_001005155 | F:CCCTCGGGTACTACACTATG R:GGCTGGGCAATCATATCC |
60 | 192 |
| GDF9 | AY_626786 | F:GAGCTCAGGACACTCTAAGCT R:CTTCTCGTGGATGATGTTCTG |
60 | 272 |
| CCNB1 | NM_001170768.1 | F:CCAACTGGTTGGTGTCACTG R:GCTCTCCGAAGAAAATGCAG |
60 | 195 |
| MTOR | XM_003127584.3 | F:AGGAGACCTCCTTTAACCAG R:ATGTACTTCCTGCACCACTC |
55 | 70 |
| RICTOR | XM_003483813.1 | F:GGTGCTAAAATTGAAAGTGG R:TGCTTGTGTCCTCTCTACCT |
55 | 82 |
| LOC100512677 | XM_003131151.3 | F:GTCACTGCCATGGAGTATCT R:CAAGTCAGCAAAGTTCTTCC |
55 | 99 |
| ATG5 | NM_001037152.1 | F:GAATATGAAGGCACACCACT R:AAATTGAGGCAAGAAGATCA |
55 | 70 |
| BECN1 | NM_001044530.1 | F:TTTTCTGGGACAACAAGTTT R:CAACCTCTTCTTTGAACTGC |
55 | 75 |
| ATG7 | NM_001190285.1 | F:GAACGGGAAGGATTTAATTT R:CAGTCAAGTCCTCCAAGAAG |
55 | 70 |
| MAP1LC3B | NM_001190290 | F:CCGAACCTTCGAACAGAGAG R:AGGCTTGGTTAGCATTGAGC |
60 | 206 |
| ATG12 | NM_001190282.1 | F:CATCCTACTAAAGGCTGTGG R:AGTCAATGAGTCCTTGGATG |
55 | 92 |
| GABARAP | NM_001190288.1 | F:CCTCCAGTACTCCTTTCCTT R:TGACAGAGATGGACAATCAA |
55 | 78 |
| GABARAPL1 | NM_001190287.1 | F:TAGGAAACGTTGAGAGGGTA R:TAACCCCGAAATGAAAATAA |
55 | 76 |
| BCL2L1 | AF216205 | F:ACTGAATCAGAAGCGGAAAC R:AAAGCTCTGATACGCTGTCC |
60 | 249 |
| BIRC5 | NM_214141 | F:CCTGGCAGCTCTACCTCAAG R:GAAAGCACAACCGGATGAAT |
60 | 233 |
| FAS | AJ001202 | F:AAGTTCCCAAGCAAGGGATT R:AATTTCCCATTGTGGAGCAG |
60 | 207 |
| CASP3 | NM_214131.1 | F:GAGGCAGACTTCTTGTATGC R:ACAAAGTGACTGGATGAACC |
55 | 93 |
| POU5F1 | NM_001113060 | F:AGTGAGAGGCAACCTGGAGA R:TCGTTGCGAATAGTCACTGC |
60 | 166 |
| NANOG | DQ447201.1 | F:GAACTTTCCAACATCCTGAA R:TTTCTGCCACCTCTTACATT |
55 | 87 |
| SOX2 | EU503117 | F:GCCCTGCAGTACAACTCCAT R:GCTGATCATGTCCCGTAGGT |
60 | 216 |
F, forward; R, reverse.