Table 1.
Defined porcine gene primers for PCR and qRT-PCR
| Gene | Abbr. | Primer sequences (5′ to3′) | Annealing temperature (°C) | GenBank accession no. |
|---|---|---|---|---|
| ZO-1 | ZO-1-f ZO-1-r |
GACTTAAAGCTGCCTCAACA GGTTTGTTTCAGGCGAAAGT |
62.0 | XM003353439.2 |
| Claudin-1 | Claudin-1-f Claudin-1-r |
TTCTGGGAGGTGCCCTACTT TGGATAGGGCCTTGGTGTTG |
59.5 | NM001244539.1 |
| Occludin | Occludin-f Occludin-r |
CCATGGCCTACTACTCGTCC CGGCACCGGTGTTGATTTAT |
59.5 | NM001163647.1 |
| ERK1 | ERK1-f ERK1-r |
TGCATTCTGGCTGAGATGCT TGAGCTGGTCCAGGTAGTGC |
55.9 | HM745137.1 |
| ERK2 | ERK2-f ERK2-r |
CCGTGACCTCAAACCTTCCA CCAACCCAAAGTCACAGATC |
59.5 | HM590585.1 |
| p90RSK | p90RSK-f p90RSK-r |
ACCTGGTGTCAAAGATGCTTC CAGTGGACGATCCAAGGATGT TCGGAAGTGTGTATCCCGTAGG GCAAGGAACACGGCTAAGTTG |
59.5 | XM003134969.3 |