Figure 1.

Identification of the integrated pPICZA::CecP4 recombinant plasmid into Pichia pastoris AOX1 gene. (A) Identification of integrated pPICZA::CecP4 by restriction enzyme digestion. Genomic DNAs were isolated from control strain with empty vector, pPICZA (C) and four transformants with pPICZA::CecP4 (T1, T2, T3, and T4) and used for restriction enzyme digestion with EcoRI and Xba I. (B) Sequences analysis of the insert CecP4 in pPICZA::CecP4. Amino acids with bold letters represent myc epitope and polyhistidine, respectively.