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. 2014 Feb;27(2):278–283. doi: 10.5713/ajas.2013.13615

Figure 2.

Figure 2

Growth curve of the CecP4 transformant and antibacterial activity of secreted recombinant CecP4 from P. pastoris. (A) Growth curve of CecP4 transformant with different dose of methanol for induction. For these experiments, CecP4 transformant (CecP4-2) and control transformant were grown in different concentrations of methanol media (1% and 2%) for the indicated time before measuring the cell growth by spectrometric analysis of the culture density. This is a representative result of two experiments (n = 2, * p<0.05). (B) Antibacterial activity of secreted recombinant CecP4 protein against Staphylococcus aureus KCTC 1621. Antibacterial activity was analyzed by agar diffusion assay using the culture supernatants for the indicated time points.