Table 1.
Gene | Primer sequences | Amplicon size (bp) |
---|---|---|
GAPDH | F. 5′-GGGTCATCATCTCTGCACCT R. 5′-GGTCATAAGTCCCTCCACGA |
177 |
FASN | F. 5′-AGGACCTCGTGAAGGCTGTGA R. 5′-CCAAGGTCTGAAAGCGAGCTG |
85 |
ACACA | F. 5′-CATCTTGTCCGAAACGTCGAT R. 5′-CCCTTCGAACATACACCTCCA |
101 |
SCD | F. 5′-TCCTGTTGTTGTGCTTCATCC R. 5′-GGCATAACGGAATAAGGTGGC |
101 |
CD36 | F. 5′-CCTCTTGGCAACCACTTTCA R. 5′-GCTTTGACACCCGAGTAACG |
113 |
FABP3 | F. 5′-GAACTCGACTCCCAGCTTGAA R. 5′-AAGCCTACCACAATCATCGAAG |
102 |
PPARG | F. 5′-CCAAATATCGGTGGGAGTCG R. 5′-ACAGCGAAGGGCTCACTCTC |
101 |
CSN1S1 | F. 5′-ACATCCTATCAAGCACCAAGGACTC R. 5′-GACGAAATGCTTTCAGCTTCCA |
192 |
STAT5 | F. 5′-AAGACCCAGACCAAGTTCGC R. 5′-AGCACCGTGGCAGTAGCAT |
422 |
MTOR | F. 5′-TGAACTGGAGGCTGATGGACAC R. 5′-TGACTGGCCAGCAGAGTAGGAA |
83 |
GAPDH = Glyceraldehyde-3-phosphate dehydrogenase; FASN = Fatty acid synthase; ACACA = Acetyl-CoA Carboxylase; SCD = Stearoyl-CoA desaturase; CD36 = Cluster of differentiation 36; FABP3 = Fatty acid-binding protein 3; PPARG = Peroxisome proliferator-activated receptor-γ; CSN1S1 = αs1-casein; STAT5 = Signal transducer and activator of transcription 5; Mtor = Mammalian target of rapamycin.