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Immobilization of LX-1 α-galactosidase on Eudragit L-100. “A” represents the amount of enzyme theoretically bound to the matrix. This is calculated by subtracting the unbound activity in the supernatant from initially added enzyme. “B” represents the expressed activity of the particular immobilized preparation, measured after incubating the immobilized enzyme with the substrate. Data were expressed as mean±standard error from three experiments.
