Table 5.

Effect of carbohydrate sources and cotton seed meal levels on volatile fatty acid concentration in rumen fluid

		CC1		CR		SEM	Significance2
		LCM	HCM	LCM	HCM		CS	CM	I
Total VFA, mmol/L
h-post feeding	0	90.8	93.7	95.4	97.1	1.24	ns	ns	ns
	4	91.7	96.7	96.8	98.8	2.04	ns	ns	ns
	mean	91.3	94.2	96.1	98.0	1.40	ns	*	ns
		--------------------- mol/100 mol ---------------------
C2, h-post feeding	0	68.6	71.9	71.6	70.2	0.75	ns	ns	ns
	4	68.1	70.4	70.2	71.5	0.87	ns	ns	ns
	mean	68.4	71.2	70.9	70.9	0.57	ns	ns	ns
C3, h-post feeding	0	22.1	19.1	19.4	21.1	0.50	ns	ns	ns
	4	21.9	20.7	20.9	20.1	0.62	ns	ns	ns
	mean	22.0	19.9	20.3	20.6	0.46	ns	ns	ns
C4, h-post feeding	0	9.2	8.9	9.0	8.7	0.19	ns	ns	ns
	4	9.7	9.0	8.9	8.4	0.21	*	ns	ns
	mean	9.5	8.9	9.0	8.6	0.20	ns	ns	ns
C2:C3, h-post feeding	0	2.7	3.3	3.7	3.3	0.31	*	ns	ns
	4	3.2	3.5	3.4	3.6	0.15	ns	ns	ns
	mean	3.0	3.4	3.6	3.5	0.10	ns	ns	ns

¹CC = Cassava chip, CR3:1 = Cassava chip+rice bran 3:1, LCM = Low cotton seed meal, HCM = High cotton seed meal.

²CS = Energy sources, CM = Cotton seed meal levels, I = ES×CM interaction.

^*p<0.05, ns = Non-significant difference, SEM = Standard error of the means.