Table 2.

Information of 20 microsatellite loci

Locus	Primer sequence (5′- 3′)	Chromosome	Allele number	Fluorescent marker	Annealing temperature (°C)	Fragment size
ILSTS029	TGTTTTGATGGAACACAGCC TGGATTTAGACCAGGGTTGG	1	9	NED	53	152–180
OarFCB20	AAATGTGTTTAAGATTCCATACAGTG GGAAAACCCCCATATATACCTATAC	2	11	6FAM	55	82–104
MAF70	CACGGAGTCACAAAGAGTCAGACC GCAGGACTCTACGGGGCCTTTGC	4	10	PET	60	142–184
MCM527	GTCCATTGCCTCAAATCAATTC AAACCACTTGACTACTCCCCAA	5	11	NED	58	151–173
ILSTS087	AGCAGACATGATGACTCAGC CTGCCTCTTTTCTTGAGAGC	6	11	PET	52	138–158
SPS113	CCTCCACACAGGCTTCTCTGACTT CCTAACTTGCTTGAGTTATTGCCC	7	9	PET	58	130–152
ILSTS005	GGAAGCAATGAAATCTATAGCC TGTTCTGTGAGTTTGTAAGC	7	8	6FAM	55	181–216
ILSTS011	GCTTGCTACATGGAAAGTGC CTAAAATGCAGAGCCCTACC	9	11	6FAM	58	268–282
SRCRSP3	CGGGGATCTGTTCTATGAAC TGATTAGCTGGCTGAATGTCC	10	7	NED	55	107–123
CSRD247	GGACTTGCCAGAACTCTGCAAT CACTGTGGTTTGTATTAGTCAGG	14	10	VIC	58	211–246
INRA063	ATTTGCACAAGCTAAATCTAACC AAACCACAGAAATGCTTGGAAG	14	9	NED	58	165–199
MAF065	AAAGGCCAGAGTATGCAATTAGGAG CCACTCCTCCTGAGAATATAACATG	15	14	VIC	55	113–147
OarFCB48	GACTCTAGAGGATCGCAAAGAACCAG GAGTTAGTACAAGGATGACAAGAGGCAC	17	7	6FAM	57	138–178
MAF209	TCATGCACTTAAGTATGTAGGATGCTG GATCACAAAAAGTTGGATACAACCGTGG	17	8	VIC	55	87–103
BMS1494	TCTGGAGCTTGCAAAAGACC AATGGATGACTCCTGGATGG	18	7	6FAM	55	227–237
SRCRSP5	GGACTCTACCAACTGAGCTACAAG TGAAATGAAGCTAAAGCAATGC	18	12	VIC	58	152–182
BM1818	AGCTGGGAATATAACCAAAGG AGTGCTTTCAAGGTCCATGC	20	7	VIC	50	200–284
OarFCB304	CCCTAGGAGCTTTCAATAAAGAATCGG CGCTGCTGTCAACTGGGTCAGGG	22	15	VIC	55	150–188
DRBP1	ATGGTGCAGCAGCAAGGTGAGCA GGGACTCAGTCTCTCTATCTCTTTG	23	16	PET	53	106–154
OarAE54	TACTAAAGAAACATGCTCCCAC AGAAACATTTATTCTTATCCTCAGTG	25	14	6FAM	56	112–134

NED: Black fluorescent; 6FAM: Blue fluorescent; PET: Red fluorescent; VIC: Green fluorescent.