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Oligonucleotide primers used in various PCRs
| Target genes | Sense and antisense primers (5′→3′) | Size of amplicons (bp) | Annealing temp. (°C) |
|---|---|---|---|
| Delta-like1 | AAATGGATTCTGCGAGGATG | 292 | 62 |
| CTGGCCATTATCAAGGTTCG | |||
| Delta-like4 | CACTTTCAGGCGGTCGTC | 337 | 62 |
| TGCAGATGACCCGGTAGG | |||
| Notch2 | CCGCAACCGAGTAACTGA | 328 | 56 |
| TGTGATGTCCCGATTGG | |||
| Jagged1 | TGGCGTCAACTCCTACAAG | 290 | 62 |
| GGGCTATGTTACAGGTCGTTC | |||
| β-actin | TGCGGGACATCAAGGAGAAG | 216 | 64 |
| AGTTGAAGGTGGTCTCGTGG |
