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. 2013 Mar;26(3):334–342. doi: 10.5713/ajas.2012.12564

Figure 1.

Figure 1.

Effect of different hormones treated for survival ability spermatozoa and acrosome reacted under hormones culture for 1, 6, 18 and 24 h. *,** Different letters within the same column represent a significant difference (p<0.05). Black arrows indicate normal acrosome detection. And white arrows indicate abnormal acrosome detection. A: Hoechst33258 analysis of sperm viability under hormones culture for 1, 6, 18 and 24 h. Stained spermatozoa were considered dead and the unstained alive. (Graph of survival ability spermatozoa), B: Effects of hormones on acrosome morphology spermatozoa culture for 6, 18 and 24 h, B-a: Percentage of acrosome morphology. FBO: Control, FFBO: FSH treated culture medium, LFBO: LH treated culture medium, LuFBO: Lutalyse treated culture medium.