. 2013 Mar;26(3):358–365. doi: 10.5713/ajas.2012.12507

Table 5.

VFA and purine concentration as influenced by different feed treatments at 12 h incubation

Item	Treatment¹						SEM²	significance
	C			EC

	S	ES	FS	S	ES	FS		CT⁴	ST⁴	CT×ST⁴
SI³	0.85	0.95	0.71	0.93	0.8	0.72

VFA concentration (mM)
Total	81.77^b	87.22^b	63.78^c	97.21^a	103.20^a	85.44^b	1.506	^**	^**	^**
Acetate	41.06^cd	42.99^bc	33.59^e	44.61^ab	45.79^a	39.10^d	0.5146	^**	^**	^**
Propionate	25.48^c	28.04^bc	18.69^d	35.40^a	38.52^a	30.65^b	0.7316	^**	^**	^**
Iso-butyrate	0.93^cd	1.06^bc	0.69^d	1.34^ab	1.52^a	0.92^cd	0.0761	^**	^**	^**
Butyrate	12.05^b	12.77^b	9.14^c	13.46^ab	14.59^a	12.55^b	0.3423	^**	^**	^**
Iso-Valerate	1.24^b	1.30^ab	0.88^d	1.23^b	1.41^a	1.04^c	0.0295	^**	^**	^**
Valerate	1.01^bc	1.06^b	0.79^c	1.16^ab	1.33^a	1.18^ab	0.052	^**	^**	^**
A:P ratio	1.61^b	1.53^b	1.8^a	1.26^c	1.19^c	1.28^c	0.0304	^**	^**	^**
Purine base (μg/ml)
0 h	9.13	9.17	8.94	8.92	8.88	8.82	0.1366	NS	NS	NS
12 h	30.09^c	33.98^b	19.52^e	38.89^a	40.15^a	27.67^d	0.2923	^**	^**	^**
24 h	33.92^a	33.42^a	29.11^b	27.86^b	27.09^b	26.70^b	0.7118	^**	^**	^*

C = Corn, EC = Enzyme treated corn, S = Soybean meal, ES = Enzyme treated soybean meal, FS = Formaldehyde treated soybean meal.

SEM means standard error of the mean.

SI = Synchrony index. SI close to 1.0 means that ruminal release of protein and energy sources is balanced appropriately compared to those having lower SI.

Significant at p<0.05.

^**

Significant at p<0.01.

NS = Not significant (p>0.05).

⁴

CT = The effect of C treatment, ST = The effect of S treatment and CT×ST = The interaction between C and S treatment.

^{a, b, c, d, e}

Indicates significant difference within the same raw (p<0.05).