Table 1.
Detail information of selected candidate reference genes for mRNA expression
| Gene | Full name | Function | GenBank accession number | Primer (5′-3′) | Reference | Length (bp) | qPCR efficiency |
|---|---|---|---|---|---|---|---|
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Carbohydrate metabolism | AF017079 | F: TCGGAGTGAACGGATTTG R: CCTGGAAGATGGTGATGG |
Martino et al., 2011 | 219 | 97.90 |
| ACTB | Beta-actin | Involved in cell motility, structure and integrity | DQ178130 | F: TCTGGCACCACACCTTCT R: GATCTGGGTCATCTTCTCAC |
Martino et al., 2011 | 114 | 101.20 |
| B2M | Beta-2-microglobulin | Ctoskeletal protein involved in cell locomotion | NM 213978 | F: TTCACACCGCTCCAGTAG R:CCAGATACATAGCAGTTCAGG |
Martino et al., 2011 | 166 | 109.30 |
| TBP | TATA binding protein | Transcription initiation from RNA polymerase II promotor | DQ178129 | F: GATGGACGTTCGGTTTAGG R: AGCAGCACAGTACGAGCAA |
Martino et al., 2011 | 124 | 101.40 |
| RPL4 | Ribosomal protein L4 | Structural constituent of ribosome | DQ845176 | F: AGGAGGCTGTTCTGCTTCTG R: TCCAGGGATGTTTCTGAAGG |
Cinar et al., 2012 | 185 | 97.8 |
| PPIA | Peptidylprolyl isomerase A (cyclophilin A) | A member of the peptidylprolyl cis-trans isomerase (PPIase) family | NM_214353 | F: CACAAACGGTTCCCAGTTTT R: TGTCCACAGTCAGCAATGGT |
Cinar et al., 2012 | 171 | 95.3 |