Table 2.
Expression levels of candidate reference genes for mRNA expression analysis with or without poly I: C stimulation
| Sample type | Experimental group | GAPDH | ACTB | B2M | TBP | RPL4 | PPIA |
|---|---|---|---|---|---|---|---|
| PBMC1 | Non-stimulation | 30.18±1.72 | 20.75±1.00a | 22.24±1.22 | 29.35±1.23 | 25.73±1.83 | 22.28±1.03 |
| Poly I:C stimulation | 30.55±1.74 | 22.15±1.29b | 22.07±1.18 | 29.38±1.15 | 25.14±1.62 | 22.33±1.00 | |
| All samples of PBMC | 30.42±1.68 | 21.68±1.36 | 22.13±1.16 | 29.37±1.14 | 25.34±0.66 | 22.32±0.98 | |
| Whole blood | Non-stimulation | 31.55±1.11 | 22.07±1.46 | 24.27±1.48 | 31.38±1.05 | 28.66±1.67 | 24.56±1.66 |
| Poly I: C stimulation | 30.95±1.64 | 22.48±0.85 | 23.53±1.34 | 31.42±1.48 | 28.83±1.97 | 24.10±1.46 | |
| All samples of whole blood | 31.15±1.48 | 22.34±1.06 | 23.78±1.39 | 31.41±1.32 | 28.77±1.82 | 24.26±1.50 | |
| All samples | 30.79±1.60 | 22.01±1.25 | 22.95±1.52 | 30.39±1.60 | 27.05±2.45 | 23.29±1.59 |
Expression levels of candidate reference genes were denoted by average±standard deviation (SD).
Gene with different superscript indicates significant difference between gene expression levels in poly I:C stimulation group and non-stimulation group by t-test (p≤0.05).
1
PBMC = Peripheral blood mononuclear cells.