FIGURE 3.

Integrity of the basement membrane (BM) is disrupted around the wound site upon nematode infection of the Drosophila larva. Viking (Collagen IV)-GFP fusion protein (protein trap) was used as a basement membrane reporter to analyze nematode infected Drosophila gut using confocal microscopy. Expression of the Viking-GFP is controlled by the endogenous promoter and enhancer elements. Note that Viking-GFP (green) signal was missing beyond the melanized area in the wound site (dark black, straight line, the area that lacks the green signal is outlined by the dashed line), which was caused by the nematode (Arefin et al., 2014; re-produced with kind permission from S. Karger AG, Basel).