Table 1.
Target gene | Sequence of primers | Allele and PCR product size (bp) |
OGG1 | F1: 5’-CAGCCCAGACCCAGTGGACTC-3’ | C allele (252 bp) |
Ser326Cys | R1: 5’-TGGCTCCTGAGCATGGCGGG-3’ | |
F2: 5’-CAGTGCCGACCTGCGCCAATG-3’ | G allele (194 bp) | |
R2: 5’-GGTAGTCACAGGGAGGCCCC-3’ | ||
XRCC1 | F1: 5’-TCCCTGCGCCGCTGCAGTTTCT-3’ | G allele (447 bp) |
Arg399Gln | R1: 5’-TGGCGTGTGAGGCCTTACCTCC-3’ | |
F2: 5’-TCGGCGGCTGCCCTCCCA-3’ | A allele (222 bp) | |
R2: 5’-AGCCCTCTGTGACCTCCCAGGC-3’ | ||
APE1 | F1: 5’-CCTACGGCATAGGTGAGACC-3’ | G allele (167 bp) |
Asp148Glu | R1: 5’-TCCTGATCATGCTCCTCC-3’ | |
F2: 5’-TCTGTTTCATTTCTATAGGCGAT-3’ | T allele (236 bp) | |
R2: 5’-GTCAATTTCTTCATGTGCCA-3’ | ||
APE1 promoter | F1: 5’-CTAACTGCCAGGGACGCCGA-3’ | T allele (136 bp) |
-141T/G | R1: 5’-ACACTGACTTAAGATTCTAACTA-3’ | |
F2: 5’-ACTGTTTTTTTCCCTCTTGCACAG-3’ | G allele (335 bp) | |
R2: 5’-TGAGCAAAAGAGCAACCCCG-3’ |
PCR: Polymerase chain reaction; OGG1: 8-oxoguanine DNA-glycosylase 1; APE1: Apurinic endonuclease 1; XRCC1: X-ray repair cross-complementing protein 1.